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- Title
A New Quantitative Fluorimetric Assay for Phagocytosis of Bacteria.
- Authors
Vray, B.; Hoebeke, J.; Saint-Guillain, M.; Leloup, R.; Strosberg, A. D.
- Abstract
We describe a new quantitative fluorimetric assay for phagocytosis of bacteria. A suspension of fluorescein-labelled bacteria (Micrococcus lysodeikticus) is mixed and incubated with phagocytes. After fixation with paraformaldehyde, the excess non-phagocytosed and adsorbed bacteria are lysed with a solution of lysozyme in phosphate-buffered saline. After washing of the phagocytes. the fluorescence of those that have ingested the labelled bacteria is measured with a spectrofluorimeter. We report results obtained with different types of phagocytes which show that this method allows sensitivity, saturation and kinetic studies and the calculation of a phagocytic index.
- Subjects
PHAGOCYTOSIS; BACTERIA; FLUORIMETRY; IMMUNE response; PHAGOCYTES; LYSOZYMES; SALINE solutions; ANALYTICAL chemistry
- Publication
Scandinavian Journal of Immunology, 1980, Vol 11, Issue 2, p147
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1980.tb00220.x