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- Title
A Chemical Approach to the Mechanism of B-Lymphocyte Activation II. The Pure Presentation of Haptens does not Inactivate B Lymphocytes.
- Authors
Vidal-Gomez, J.
- Abstract
Dinitrophenyl (DNP)-lysine-polymethylmethacrylate and DNP-cellulose conjugates do not irreversibly inactivate anti-DNP antigen-sensitive cells, regardless of the dose (up to 10 mg) or persistence of the stimulation (up to 2 weeks). Since these conjugates constitute pure hapten presentations, it is concluded that the pure hapten presentation to B lymphocyte does not irreversibly inactivate them. When murine spleen cells are culture with Escherichia coli lipopolysaccharide (LPS) and (non-immunogenic) DNP-lysine-polymethylmethacrylate or (non-immunogenic) DNP-cellulose conjugates, an anti-DNP immune response occurs. However, replacement of DNP-lysine-polymethylmethacrylate with polymethylmethacrylate, or DNP-cellulose with cellulose, also results in a similar anti-DNP response. It is consequently concluded that the anti-DNP response elicited by DNP-Ficoll is, upon exhaustive testing, carrier-dependent. This implies that the mechanism of DNP-Ficoli immunogenicity is not two cooperative signals passed on to B lymphocytes via the hapten DNP. These results argue against any two-signal model of B-lymphocyte activation.
- Subjects
HAPTENS; ANTIGENS; B cells; LYMPHOCYTES; POLYMETHYLMETHACRYLATE; DINITROBENZENES
- Publication
Scandinavian Journal of Immunology, 1978, Vol 8, Issue 4, p323
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1978.tb00525.x