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- Title
Responses of Alloantigen-Primed Lymphocytes in Vitro.
- Authors
Corley, R. B.
- Abstract
Maximal proliferative responses were observed at lower responder cell densities in alloantigen-primed lymphocyte populations when restimulatd with the specific priming cell than when restimulated with other allngencic cells. This relationship was due to the larger number of lymphocytes within the primed population which responded to the specific sensitizing cell than to unrelated stimulator cells. As a result, at high responder cell densities, stimulator ceils unrelated to the specific sensitizing cells elicited a disproportionately high response relative to the response stimulated by the specific cells. Restimulatioa of decreasing number of primed lymphocytes gave a mote accurate representation of the stimulatory capacity of different cells. Because primed populations contain a larger number of lymphocytes that respond to the specific stimulator than to other stimulators, responses of primed lymphocytes could be made operationally monospecific. Limiting dilution analysis demonstrated that low numbers of primed cells were stimulated by the specific sensitizing cells but not by unrelated stimulator cells.
- Subjects
CELL proliferation; LYMPHOCYTES; B cells; LEUCOCYTES; POPULATION; DILUTION
- Publication
Scandinavian Journal of Immunology, 1977, Vol 6, Issue 5, p547
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1977.tb02119.x