We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Expression and Activity Analysis of Fructosyltransferase from Aspergillus oryzae.
- Authors
Guan, Lihong; Chen, Liping; Chen, Yongsen; Zhang, Nu; Han, Yawei
- Abstract
The fructosyltransferase gene was isolated and cloned from Aspergillus oryzae. The gene was 1368 bp, which encoded a protein of 455 amino acids. To analyze the activity of the expressed fructosyltransferase, the pET32a-fructosyltransferase recombined plasmid was transformed into Escherichia coli BL21. The fructosyltransferase gene was successfully expressed by Isopropyl-β-d-thiogalactoside (IPTG) induction. The molecular weight of the expression protein was about 45 kDa. The optimal conditions of protein expression were 25 °C, 0.1 mM IPTG, and 8 h of inducing time. The optimal concentration of urea dealing with inclusion body was 2.5 M. The expressed protein exhibited a strong fructosyl transfer activity. These results showed that the expressed fructosyltransferas owned transferase activity, and could catalyze the synthesis of sucrose-6-acetate.
- Subjects
KOJI; FRUCTOSYLTRANSFERASES; PROTEIN expression; ESCHERICHIA coli; CHEMICAL synthesis
- Publication
Protein Journal, 2017, Vol 36, Issue 4, p352
- ISSN
1572-3887
- Publication type
Article
- DOI
10.1007/s10930-017-9725-y