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- Title
In vitro Translation of mRNA from Rat Peritoneal and Intestinal Mucosal Mast Cells.
- Authors
Imai, Toru; Fujimaki, Hidekazu; Abe, Tatsuya; Befus, Dean
- Abstract
We isolated RNA from in vivo-derived rat peritoneal (PMC) and intestinal mucosal mast cells (IMMC) and assessed the in vitro translation products of these two mast cell types using rabbit reticulocyte lysate and wheat germ systems. The translated polypeptides were analyzed by one- and two-dimensional gel electrophoresis. Fluorographic analyses established that polypeptides with a spectrum of molecular size and pI were produced by both PMC and IMMC. Immunoprecipitation studies with anti-rat mast cell protease (RMCP) I antibodies detected a band at 31 kD from products of PMC RNA assessed by one-dimensional electrophoresis, whereas anti-RMCP II antibodies precipitated a band of 27 kD from products of IMMC RNA. This is the first report of translation products of IMMC and of the translated precursors of RMCP I and RMCP II. Copyright © 1993 S. Karger AG, Basel
- Publication
International Archives of Allergy & Immunology, 1993, Vol 102, Issue 1, p26
- ISSN
1018-2438
- Publication type
Article
- DOI
10.1159/000236547