We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton.
- Authors
Xu, Ke; Babcock, Hazen P; Zhuang, Xiaowei
- Abstract
By combining astigmatism imaging with a dual-objective scheme, we improved the image resolution of stochastic optical reconstruction microscopy (STORM) and obtained <10-nm lateral resolution and <20-nm axial resolution when imaging biological specimens. Using this approach, we resolved individual actin filaments in cells and revealed three-dimensional ultrastructure of the actin cytoskeleton. We observed two vertically separated layers of actin networks with distinct structural organizations in sheet-like cell protrusions.
- Subjects
CYTOSKELETON; ACTIN; ASTIGMATISM; MICROSCOPY; CELLS; MEDICAL imaging systems
- Publication
Nature Methods, 2012, Vol 9, Issue 2, p185
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1841