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- Title
Magnetic torque tweezers: measuring torsional stiffness in DNA and RecA-DNA filaments.
- Authors
Lipfert, Jan; Kerssemakers, Jacob W. J.; Jager, Tessa; Dekker, Nynke H.
- Abstract
We introduce magnetic torque tweezers, which enable direct single-molecule measurements of torque. Our measurements of the effective torsional stiffness C of dsDNA indicated a substantial force dependence, with C = ?40 nm at low forces up to C = ?100 nm at high forces. The initial torsional stiffness of RecA filaments was nearly twofold larger than that for dsDNA, yet at moderate torques further build-up of torsional strain was prevented.
- Subjects
DNA; TORSIONAL vibration; TORQUE; MAGNETIC tweezers; MAGNETIC instruments
- Publication
Nature Methods, 2010, Vol 7, Issue 12, p977
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1520