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- Title
In situ detection and genotyping of individual mRNA molecules.
- Authors
Larsson, Chatarina; Grundberg, Ida; Söderberg, Ola; Nilsson, Mats
- Abstract
Increasing knowledge about the heterogeneity of mRNA expression within cell populations highlights the need to study transcripts at the level of single cells. We present a method for detection and genotyping of individual transcripts based on padlock probes and in situ target-primed rolling-circle amplification. We detect a somatic point mutation, differentiate between members of a gene family and perform multiplex detection of transcripts in human and mouse cells and tissue.
- Subjects
FLUORESCENCE in situ hybridization; MESSENGER RNA; HETEROGENEITY; CELL populations; GENE amplification
- Publication
Nature Methods, 2010, Vol 7, Issue 5, p395
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1448