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- Title
Adipose-derived mesenchymal stem cell protects kidneys against ischemia-reperfusion injury through suppressing oxidative stress and inflammatory reaction.
- Authors
Yen-Ta Chen; Cheuk-Kwan Sun; Yu-Chun Lin; Li-Teh Chang; Yung-Lung Chen; Tzu-Hsien Tsai; Sheng-Ying Chung; Sarah Chua; Ying-Hsien Kao; Chia-Hung Yen; Pei-Lin Shao; Kuan-Cheng Chang; Steve Leu; Hon-Kan Yip; Chen, Yen-Ta; Sun, Cheuk-Kwan; Lin, Yu-Chun; Chang, Li-Teh; Chen, Yung-Lung; Tsai, Tzu-Hsien
- Abstract
<bold>Background: </bold>Reactive oxygen species are important mediators exerting toxic effects on various organs during ischemia-reperfusion (IR) injury. We hypothesized that adipose-derived mesenchymal stem cells (ADMSCs) protect the kidney against oxidative stress and inflammatory stimuli in rat during renal IR injury.<bold>Methods: </bold>Adult male Sprague-Dawley (SD) rats (n = 24) were equally randomized into group 1 (sham control), group 2 (IR plus culture medium only), and group 3 (IR plus immediate intra-renal administration of 1.0 × 106 autologous ADMSCs, followed by intravenous ADMSCs at 6 h and 24 h after IR). The duration of ischemia was 1 h, followed by 72 hours of reperfusion before the animals were sacrificed.<bold>Results: </bold>Serum creatinine and blood urea nitrogen levels and the degree of histological abnormalities were markedly lower in group 3 than in group 2 (all p < 0.03). The mRNA expressions of inflammatory, oxidative stress, and apoptotic biomarkers were lower, whereas the anti-inflammatory, anti-oxidative, and anti-apoptotic biomarkers were higher in group 3 than in group 2 (all p < 0.03). Immunofluorescent staining showed a higher number of CD31+, von Willebrand Factor+, and heme oxygenase (HO)-1+ cells in group 3 than in group 2 (all p < 0.05). Western blot showed notably higher NAD(P)H quinone oxidoreductase 1 and HO-1 activities, two indicators of anti-oxidative capacity, in group 3 than those in group 2 (all p < 0.04). Immunohistochemical staining showed higher glutathione peroxidase and glutathione reductase activities in group 3 than in group 2 (all p < 0.02)<bold>Conclusion: </bold>ADMSC therapy minimized kidney damage after IR injury through suppressing oxidative stress and inflammatory response.
- Subjects
OXIDATIVE stress; REACTIVE oxygen species; ISCHEMIA; REPERFUSION injury; STEM cells
- Publication
Journal of Translational Medicine, 2011, Vol 9, Issue Suppl 1, p51
- ISSN
1479-5876
- Publication type
journal article
- DOI
10.1186/1479-5876-9-51