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- Title
Budesonide ameliorates lung injury induced by large volume ventilation.
- Authors
Ying-Nan Ju; Kai-Jiang Yu; Guo-Nian Wang; Ju, Ying-Nan; Yu, Kai-Jiang; Wang, Guo-Nian
- Abstract
<bold>Background: </bold>Ventilation-induced lung injury (VILI) is a health problem for patients with acute respiratory dysfunction syndrome. The aim of this study was to investigate the effectiveness of budesonide in treating VILI.<bold>Methods: </bold>Twenty-four rats were randomized to three groups: a ventilation group, ventilation/budesonide group, and sham group were ventilated with 30 ml/kg tidal volume or only anesthesia for 4 hor saline or budesonide airway instillation immediately after ventilation. The PaO2/FiO2and wet-to-dry weight ratios, protein concentration, neutrophil count, and neutrophil elastase levels in bronchoalveolar lavage fluid (BALF) and the levels of inflammation-related factors were examined. Histological evaluation of and apoptosis measurement inthe lung were conducted.<bold>Results: </bold>Compared with that in the ventilation group, the PaO2/FiO2 ratio was significantly increased by treatment with budesonide. The lung wet-to-dry weight ratio, total protein, neutrophil elastase level, and neutrophilcount in BALF were decreased in the budesonide group. The BALF and plasma tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, intercellular adhesion molecule (ICAM)-1, and macrophage inflammatory protein (MIP)-2 levels were decreased, whereas the IL-10 level was increased in the budesonide group. The phosphorylated nuclear factor (NF)-kBlevels in lung tissue were inhibited by budesonide. The histological changes in the lung and apoptosis were reduced by budesonide treatment. Bax, caspase-3, and cleaved caspase-3 were down-regulated, and Bcl-2 was up-regulated by budesonide.<bold>Conclusions: </bold>Budesonide ameliorated lung injury induced by large volume ventilation, likely by improving epithelial permeability, decreasing edema, inhibiting local and systemic inflammation, and reducing apoptosis in VILI.
- Subjects
BUDESONIDE; LUNG injuries; ARTIFICIAL respiration; BRONCHOALVEOLAR lavage; APOPTOSIS; NEUTROPHILS; THERAPEUTIC use of glucocorticoids; ANIMAL experimentation; ANTIGENS; BODY fluids; COMPARATIVE studies; CYTOKINES; INTERLEUKIN-1; INTERLEUKINS; LUNGS; RESEARCH methodology; MEDICAL cooperation; PROTEINS; RATS; RESEARCH; RESPIRATORY measurements; STATISTICAL sampling; TUMOR necrosis factors; DNA-binding proteins; MECHANICAL ventilators; EVALUATION research; LEUKOCYTE count; THERAPEUTICS
- Publication
BMC Pulmonary Medicine, 2016, Vol 16, p1
- ISSN
1471-2466
- Publication type
journal article
- DOI
10.1186/s12890-016-0251-z