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- Title
Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p.
- Authors
Pan, Dongqing; Nakatsu, Toru; Kato, Hiroaki
- Abstract
Appropriate targeting of matrix proteins to peroxisomes is mainly directed by two types of peroxisomal targeting signals, PTS1 and PTS2. Although the basis of PTS1 recognition has been revealed by structural studies, that of PTS2 recognition remains elusive. Here we present the crystal structure of a heterotrimeric PTS2-recognition complex from Saccharomyces cerevisiae, containing Pex7p, the C-terminal region of Pex21p and the PTS2 of the peroxisomal 3-ketoacyl-CoA thiolase. Pex7p forms a β-propeller structure and provides a platform for cooperative interactions with both the amphipathic PTS2 helix and Pex21p. The C-terminal region of Pex21p directly covers the hydrophobic surfaces of both Pex7p and PTS2, and the resulting hydrophobic core is the primary determinant of stable complex formation. Together with in vivo and in vitro functional assays of Pex7p and Pex21p variants, our findings reveal the molecular mechanism of PTS2 recognition.
- Subjects
EXTRACELLULAR matrix proteins; PEROXISOMES; CRYSTAL structure; SACCHAROMYCES cerevisiae; C-terminal binding proteins; HYDROPHOBIC surfaces; MOLECULAR recognition; BEHAVIOR
- Publication
Nature Structural & Molecular Biology, 2013, Vol 20, Issue 8, p987
- ISSN
1545-9993
- Publication type
Article
- DOI
10.1038/nsmb.2618