We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Replication of Norovirus in Cell Culture Reveals a Tropism for Dendritic Cells and Macrophages.
- Authors
Wobus, Christiane E.; Karst, Stephanie M.; Thackray, Larissa B.; Kyeong-Ok Chang; Sosnovtsev, Stanislav V.; Belliot, Gaë; Krug, Anne; Mackenzie, Jason M.; Green, Kim Y.; Virgin IV, Herbert W.
- Abstract
Noroviruses are understudied because these important enteric pathogens have not been cultured to date. We found that the norovirus murine norovirus 1(MNV-1) infects macrophage-like cells in vivo and replicates in cultured primary dendritic cells and macrophages. MNV-1 growth was inhibited by the interferon-α;β receptor and STAT-1, and was associated with extensive rearrangements of intracellular membranes. An amino acid substitution in the capsid protein of serially passaged MNV-1 was associated with virulence attenuation in vivo. This is the first report of replication of a norovirus in cell culture. The capacity of MNV-1 to replicate in a STAT-1 -regulated fashion and the unexpected tropism of a norovirus for cells of the hematopoietic lineage provide important insights into norovirus biology.
- Subjects
CELL culture; RETROVIRUSES; CELLS; PATHOGENIC microorganisms; LYMPHOID tissue; PROTEINS
- Publication
PLoS Biology, 2004, Vol 2, Issue 12, p2076
- ISSN
1544-9173
- Publication type
Article
- DOI
10.1371/journal.pbio.0020432