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- Title
Development and validation of a liquid chromatography–tandem mass spectrometry assay for the simultaneous quantification of buprenorphine, norbuprenorphine, and metabolites in human urine.
- Authors
Kacinko, Sherri L.; Concheiro-Guisan, Marta; Shakleya, Diaa M.; Huestis, Marilyn A.
- Abstract
A liquid chromatography–tandem mass spectrometry method for the simultaneous quantification of buprenorphine (BUP), norbuprenorphine (NBUP), buprenorphine glucuronide (BUP-Gluc), and norbuprenorphine glucuronide (NBUP-Gluc) in human urine was developed and fully validated. Extensive endogenous and exogenous interferences were evaluated and limits of quantification were identified empirically. Analytical ranges were 5–1,000 ng/mL for BUP and BUP-Gluc and 25–1,000 ng/mL for NBUP and NBUP-Gluc. Intra-assay and interassay imprecision were less than 17% and recovery was 93–116%. Analytes were stable at room temperature, at 4 °C, and for three freeze–thaw cycles. This accurate and precise assay has sufficient sensitivity and specificity for urine analysis of specimens collected from individuals treated with BUP for opioid dependence.
- Subjects
BUPRENORPHINE; URINE; LIQUID chromatography; TANDEM mass spectrometry; OPIOID abuse
- Publication
Analytical & Bioanalytical Chemistry, 2008, Vol 392, Issue 5, p903
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-008-2326-z