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- Title
The genetic characterization of self-incompatibility in a Brassica napus line with promising breeding potential.
- Authors
Gao, Changbin; Ma, Chaozhi; Zhang, Xingguo; Li, Fupeng; Zhang, Jianfeng; Zhai, Wen; Wang, Yuanyuan; Tu, Jinxing; Shen, Jinxiong; Fu, Tingdong
- Abstract
The aim of this study was to study hybrid breeding through the self-incompatibility (SI) pollination control system. To this end, we characterized the SI of a Brassica napus line 'S-1300'. 'S-1300' was found to have two class II S haplotypes, namely BnS- 1300 and BnS- 6 located on the A and C genome, respectively. A self-compatible line '8400' had one class I S haplotype ( BnS- 1) on the A genome and one class II S haplotype ( BnS- 6) on the C genome. Genetic analysis revealed that SI in 'S-1300' was recessive to SC (self-compatibility) in '8400' and controlled by BnS- 1300 on the A genome. BnSP11- 1300 and BnSRK- 1300 were cloned by the homology-based candidate gene method and found to share 100 % identity of coding sequence with BrS- 60. The expression of BnSP11- 1300 was detected only in 'S-1300', while the transcripts of BnSRK- 1300 were detected in both 'S-1300' and 'S-1300 × 8400', which further confirmed that the class II S haplotype BnS- 1300 controls the SI in 'S-1300'. The potential of using 'S-1300' in research directed at elucidating the SC mechanism and in hybrid breeding of B. napus was discussed.
- Subjects
PLANT self-incompatibility; BRASSICA; PLANT breeding; HAPLOTYPES; POLLINATION; GENE expression in plants; PLANT hybridization
- Publication
Molecular Breeding, 2013, Vol 31, Issue 2, p485
- ISSN
1380-3743
- Publication type
Article
- DOI
10.1007/s11032-012-9805-9