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- Title
Enhanced biomass and oxidative stress tolerance of Synechococcus elongatus PCC 7942 overexpressing the DHAR gene from Brassica juncea.
- Authors
Kim, Young-Saeng; Kim, Il-Sup; Boyd, Joseph; Taton, Arnaud; Golden, James; Yoon, Ho-Sung
- Abstract
Objectives: To improve the oxidative stress tolerance, biomass yield, and ascorbate/dehydroascorbate (AsA/DHA) ratio of Synechococcus elongatus PCC 7942 in the presence of HO, by heterologous expression of the dehydroascorbate reductase (DHAR) gene from Brassica juncea ( BrDHAR). Results: Under HO stress, overexpression of BrDHAR in the transgenic strain (BrD) of S. elongatus greatly increased the AsA/DHA ratio. As part of the AsA recycling system, the oxidative stress response induced by reactive oxygen species was enhanced, and intracellular HO level decreased. In addition, under HO stress conditions, the BrD strain displayed increased growth rate and biomass, as well as higher chlorophyll content and deeper pigmentation than did wild-type and control strains. Conclusion: By maintaining the AsA pool and redox homeostasis, the heterologous expression of BrDHAR increased S. elongatus tolerance to HO stress, improving the biomass yield under these conditions. The results suggest that the BrD strain of S. elongatus, with its ability to attenuate the deleterious effects of ROS caused by environmental stressors, could be a promising platform for the generation of biofuels and other valuable bioproducts.
- Subjects
OXIDATIVE stress; BIOMASS; SYNECHOCOCCUS elongatus; BRASSICA juncea; GENETIC overexpression; BACTERIA
- Publication
Biotechnology Letters, 2017, Vol 39, Issue 10, p1499
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-017-2382-6