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- Title
Insight into transketolase of Pyropia haitanensis under desiccation stress based on integrative analysis of omics and transformation.
- Authors
Shi, Jianzhi; Wang, Wenlei; Lin, Yinghui; Xu, Kai; Xu, Yan; Ji, Dehua; Chen, Changsheng; Xie, Chaotian
- Abstract
Background: Pyropia haitanensis, distributes in the intertidal zone, can tolerate water losses exceeding 90%. However, the mechanisms enabling P. haitanensis to survive harsh conditions remain uncharacterized. To elucidate the mechanism underlying P. haitanensis desiccation tolerance, we completed an integrated analysis of its transcriptome and proteome as well as transgenic Chlamydomonas reinhardtii carrying a P. haitanensis gene. Results: P. haitanensis rapidly adjusted its physiological activities to compensate for water losses up to 60%, after which, photosynthesis, antioxidant systems, chaperones, and cytoskeleton were activated to response to severe desiccation stress. The integrative analysis suggested that transketolase (TKL) was affected by all desiccation treatments. Transgenic C. reinhardtii cells overexpressed PhTKL grew better than the wild-type cells in response to osmotic stress. Conclusion: P. haitanensis quickly establishes acclimatory homeostasis regarding its transcriptome and proteome to ensure its thalli can recover after being rehydrated. Additionally, PhTKL is vital for P. haitanensis desiccation tolerance. The present data may provide new insights for the breeding of algae and plants exhibiting enhanced desiccation tolerance.
- Subjects
BANGIALES; CHLAMYDOMONAS; PROTEOMICS; OSMOTIC pressure; INTERTIDAL zonation; CHLAMYDOMONAS reinhardtii; PLANT breeding; PSYCHOLOGICAL stress
- Publication
BMC Plant Biology, 2019, Vol 19, Issue 1, pN.PAG
- ISSN
1471-2229
- Publication type
Article
- DOI
10.1186/s12870-019-2076-4