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- Title
Laser microdissection and cryogenic nuclear magnetic resonance spectroscopy: an alliance for cell type-specific metabolite profiling.
- Authors
Schneider, B.; Hölscher, D.
- Abstract
Laser microdissection was used as a tool to harvest secretory cavities (SC) from leaves of Dilatris pillansii Barker (Haemodoraceae) and from leaves and flowers of herbarium specimens of Dilatris corymbosa Berg. and Dilatris viscosa L. Cryogenic 1H NMR spectroscopy and HPLC analysis of microdissected samples indicated specific accumulation of methoxyphenylphenalenones in the SC. The structures of two novel and a known natural product in the secretory tissue were confirmed by comparison with authentic compounds isolated from rhizomes and roots from which further phenylphenalenones and phenylphenalenone glucosides were isolated and identified by spectroscopic methods. How it will be possible to use the LMD technique to localize natural products in specific plant cell populations is also discussed.
- Subjects
MICRODISSECTION; SPECTRUM analysis; HAEMODORACEAE; NUCLEAR magnetic resonance spectroscopy; HIGH performance liquid chromatography; BOTANICAL specimens; PLANT cells &; tissues; GLUCOSIDES; PLANT anatomy
- Publication
Planta: An International Journal of Plant Biology, 2007, Vol 225, Issue 3, p763
- ISSN
0032-0935
- Publication type
Article
- DOI
10.1007/s00425-006-0404-z