We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Interaction of Fibronectin with Human Colon Adenocarcinoma Cells: Effect on the in vivo Tumorigenic Capacity.
- Authors
López-Conejo, M. Teresa; Olmo, Nieves; Turnay, Javier; López de Silanes, Isabel; Lizarbe, M. Antonia
- Abstract
Fibronectin (FN) modulates the behavior of the poorly differentiated, human colon adenocarcinoma-derived BCS-TC2 cells by promoting adhesion through the α[sub 5] β[sub 1] -integrin, as this effect is blocked by anti-α[sub 5] and β[sub 1 ] chain antibodies. BCS-TC2 cells are not tumorigenic in vivo, but are able to form tumors when coinjected with FN in nude mice. From these tumors, a tumorigenic cell subline (BCS-TC2.FN) was established. In vivo passaging of BCS-TC2.FN cells in the absence of FN allowed the selection of another tumorigenic subline (BCS-TC2.FN2). The new sublines are characterized by: (1) increased differentiation, (2) slightly higher adhesion to FN, and (3) a higher uptake of [[sup 3] H]thymidine, less dependent on the presence of serum or FN. No significant modifications in α[sub 5] -chain surface levels were observed in the tumor-derived sublines, suggesting that the amount of α[sub 5] β[sub 1] -integrin is not related to tumorigenicity. Within the heterogeneous parental cells, FN seems to favor the selection of a cell subpopulation that presents phenotypic and genotypic alterations that are stably maintained throughout in vitro culture and in vivo passaging. These cell lines constitute a model system that may help to extend our knowledge on the events underlying tumor progression and malignancy of colorectal cancer, and the influence of extracellular matrix components and their receptors in these processes.Copyright © 2002 S. Karger AG, Basel
- Subjects
FIBRONECTINS; COLON (Anatomy); ADENOCARCINOMA; CANCER cells; CELLULAR pathology; EXTRACELLULAR matrix; CELL differentiation; INTEGRINS
- Publication
Oncology, 2002, Vol 62, Issue 4, p371
- ISSN
0030-2414
- Publication type
Article
- DOI
10.1159/000065070