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- Title
Binding of Glutathione by Rat Liver Cytosol.
- Authors
Sugiyama, Y.; Kaplowitz, N.
- Abstract
Glutathione (GSH) binding to rat liver cytosol at two different protein concentrations and a range of GSH concentrations was determined using rapid ultrafiltration. Two binding sites and nonspecific binding were determined by computer fit of the data. The high-affinity site had a similar affinity and capacity for GSH as that of the GSH S-transferases. Using the converged parameters and an estimation of cytosolic protein content of the intact liver, simulation of the GSH-free fraction and the contribution and degree of saturation of the high-affinity binding site were estimated over a broad range of GSH concentrations. The findings predict that 70-75% of cytosol GSH is free and that the high-affinity site is saturated with GSH in the physiologic range. Copyright © 1984 S. Karger AG, Basel
- Publication
Pharmacology, 1984, Vol 28, Issue 2, p61
- ISSN
0031-7012
- Publication type
Article
- DOI
10.1159/000137945