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- Title
Second naturally occurring β-galactosidase in E. coli.
- Authors
HARTL, DANIEL L.; HALL, BARRY G.
- Abstract
SEVERAL studies have shown that the intestinal mucosa of various mammals contains at least two β-galactosidases1-5. One of these has a primary specificity for lactose; the other has a primary specificity for synthetic β-galactosides such as o-Nitrophenyl β-D-galactopyranoside (ONPG). Since strains of Escherichia coli K12 bearing deletions of the lac Z gene have a very small residual activity toward ONPG, there must also be a second β-galactosidase in E. coli. We report here that E. coli K12 does indeed possess a second β-galactosidase. Although this enzyme has virtually no activity toward lactose, its synthesis is induced by lactose.
- Publication
Nature, 1974, Vol 248, Issue 5444, p152
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/248152a0