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- Title
Identification of novel Z/W chromosome-specific markers from the giant freshwater prawn Macrobrachium rosenbergii.
- Authors
Xue Liu; Lingxia Zhou; Biyun Luo; Hongli Qian; Baoqing Ye; Keyi Ma; Gaofeng Qiu
- Abstract
The giant freshwater prawn (Macrobrachium rosenbergii) is a commercially cultured species in China. A sex chromosome-specific marker is crucial for sex identification and distinguishing sex chromosomes. Here, based on the results of reduced-representation genome sequencing and genome survey of M. rosenbergii, we identified steady sex-linked markers. Further nucleotide alignment analysis revealed 83.42% nucleotide similarity between the amplified W- and Z-chromosome fragments. qPCR confirmed that Z chromosome-specific fragments were present in males approximately twice as often compared to females. Similarly, the W chromosome-specific fragments were present in super females approximately twice as often compared to females. Our findings elucidate the ZW/ZZ sex determination type and contribute to exploring sex-related or sex-determining genes present on the sex chromosomes of M. rosenbergii.
- Subjects
CHINA; MACROBRACHIUM rosenbergii; SEX chromosomes; POLYMERASE chain reaction; NUCLEOTIDES
- Publication
Aquaculture & Fisheries (2096-1758), 2023, Vol 8, Issue 5, p509
- ISSN
2096-1758
- Publication type
Article
- DOI
10.1016/j.aaf.2022.01.003