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- Title
IN VITRO EFFECT OF IRON OXIDE NANOPARTICLES ON XANTHINE OXIDASE ACTIVITY.
- Authors
Hooda, Ritu; Sharma, Minakshi
- Abstract
Recently, the boom of metal oxide nanoparticles synthesis and its biomedical applications have led to emphasize on the possible impact of these nanoparticles on bio-macromolecules. In this study, we studied the impact of green Fe2O3NPs and chemically synthesized (Commercial), on xanthine oxidase (XO) activity by addition of CuO-NPs in aqueous suspension (1-100µg/ ml at an interval of 10µg) in the reaction mixture of its spectroscopic assay. The XO assay was also performed in vivo using fresh human serum in place of XO. The XO activity was decreased, as the concentration of Fe2O3NPs increased. Km value of XO was increased upon addition of both types of Fe2O3NPs indicating the lower affinity of enzyme towards the substrate in presence of Fe2O3NPs. Our results provide insight into the interaction of Fe2O3NPs with enzyme, which can be important for further applicability of nanoparticles in biomedical applications.
- Subjects
IRON oxide nanoparticles; XANTHINE oxidase; HOMEOSTASIS; METALLOPROTEINS; DIETHYLDITHIOCARBAMATE
- Publication
Plant Archives (09725210), 2020, Vol 20, Issue 1, p2114
- ISSN
0972-5210
- Publication type
Article