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- Title
Anti-Virulence Properties of Plant Species: Correlation between In Vitro Activity and Efficacy in a Murine Model of Bacterial Infection.
- Authors
Díaz-Núñez, José Luis; Pérez-López, Macrina; Espinosa, Norma; Campos-Hernández, Nayelli; García-Contreras, Rodolfo; Díaz-Guerrero, Miguel; Cortes-López, Humberto; Vázquez-Sánchez, Monserrat; Quezada, Héctor; Martínez-Vázquez, Mariano; Soto-Hernández, Ramón Marcos; Burgos-Hernández, Mireya; González-Pedrajo, Bertha; Castillo-Juárez, Israel
- Abstract
Several plant extracts exhibit anti-virulence properties due to the interruption of bacterial quorum sensing (QS). However, studies on their effects at the preclinical level are scarce. Here, we used a murine model of abscess/necrosis induced by Pseudomonas aeruginosa to evaluate the antipathogenic efficacy of 24 plant extracts at a sub-inhibitory concentration. We analyzed their ability to inhibit QS-regulated virulence factors such as swarming, pyocyanin production, and secretion of the ExoU toxin via the type III secretion system (T3SS). Five of the seven extracts with the best anti-pathogenic activity reduced ExoU secretion, and the extracts of Diphysa americana and Hibiscus sabdariffa were identified as the most active. Therefore, the abscess/necrosis model allows identification of plant extracts that have the capacity to reduce pathogenicity of P. aeruginosa. Furthermore, we evaluated the activity of the plant extracts on Chromobacterium violaceum. T3SS (∆escU) and QS (∆cviI) mutant strains were assessed in both the abscess/necrosis and sepsis models. Only the ∆escU strain had lower pathogenicity in the animal models, although no activity of plant extracts was observed. These results demonstrate differences between the anti-virulence activity recorded in vitro and pathogenicity in vivo and between the roles of QS and T3S systems as virulence determinants.
- Publication
Microorganisms, 2021, Vol 9, Issue 12, p1
- ISSN
2076-2607
- Publication type
Article
- DOI
10.3390/microorganisms9122424