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- Title
Emulsion PCR (ePCR) as a Tool to Improve the Power of DGGE Analysis for Microbial Population Studies.
- Authors
Iacumin, Lucilla; Cecchini, Francesca; Vendrame, Marco; Comi, Giuseppe
- Abstract
To the authors' knowledge, this is the first report of the use of emulsion-Polymerase chain reaction (e-PCR) coupled with denaturing gradient gel electrophoresis (DGGE) analysis. In the present work the effectiveness of ePCR in improving the power of the DGGE technique for microbial population studies was tested. Our results indicated that ePCR results in uniform amplification of several DNA molecules, overcoming the major limitations of conventional PCR, such as preferential amplification and DNA concentration dependence. Moreover, ePCR-DGGE resulted in higher sensitivity when compared to conventional PCR-DGGE methods used for studying microbial populations in a complex matrix. In fact, compared to conventional PCR, the DGGE profiles of ePCR products permitted the detection of a higher number of the species that were present in the tested sample.
- Subjects
DENATURING gradient gel electrophoresis; MICROORGANISM populations; GENE amplification; POWER tools; COMPLEX matrices; BACTERIAL ecology
- Publication
Microorganisms, 2020, Vol 8, Issue 8, p1099
- ISSN
2076-2607
- Publication type
Article
- DOI
10.3390/microorganisms8081099