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- Title
Copy number aberrations using multicolour fluorescence in situ hybridization ( FISH) for prognostication in non-muscle-invasive bladder cancer ( NIMBC).
- Authors
Matsuyama, Hideyasu; Ikemoto, Kenzo; Eguchi, Satoshi; Oga, Atsunori; Kawauchi, Shigeto; Yamamoto, Yoshiaki; Kawai, Yoshihisa; Matsumoto, Hiroaki; Hara, Takahiko; Nagao, Kazuhiro; Sakano, Shigeru; Sasaki, Kohsuke
- Abstract
Objective To investigate if detection of copy number aberrations of chromosomes 3, 7, 9p21, and 17 using multicolour fluorescence in situ hybridization ( FISH) predicts patient outcome in non-muscle-invasive bladder cancer ( NMIBC)., Patients and Methods In all, 118 bladder wash samples were prospectively collected from patients who underwent transurethral resection of bladder tumour (median age 50.5 years, male/female: 91/27, tumour grade 1/2/3: 18/52/42, stage pTis/ Ta/ T1: 8/62/42) from 2007 to 2010., The 118 samples were analysed using the Uro Vysion® kit to detect the copy numbers of chromosomes 3, 7, 9p21, and 17., The variant fraction ( VF; the sum of the non-modal copy number fraction of each chromosome) was defined as abnormal when the percentage was ≥16%. The percentage deletion of 9p21 (fraction of null or one copy number of the 9p21 locus) was defined as abnormal when the percentage was ≥12%., Maffezzini risk criteria were also analysed in our cohorts., Results There was recurrence in 57 (48.3%) patients and disease progression in 12 (10.1%), with a median follow-up of 35.7 months., Multivariate analysis showed that the percentage 9p21 loss (>12%) was an independent prognostic factor for recurrence ( P < 0.001, odds ratio [ OR] 3.24, 95% confidence interval [ CI] 1.85-5.62)., For disease progression, tumour grade, positive urine cytology, concurrent carcinoma in situ, and a mean VF of >16% were significant prognostic factors in univariate analysis. In multivariate analysis, a mean VF of >16% was a prognostic factor for disease progression ( P = 0.048, OR 6.07, 95% CI 1.02-57.45)., Conclusions Multicolour- FISH analysis using a commercially available kit could be a powerful tool not only for diagnosis, but also for prognostication in patients with NMIBC.
- Subjects
CANCER patients; IN situ hybridization; FLUORESCENCE in situ hybridization; BLADDER cancer
- Publication
BJU International, 2014, Vol 113, Issue 4, p662
- ISSN
1464-4096
- Publication type
Article
- DOI
10.1111/bju.12232