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- Title
Understanding the characteristics of phytofabricated silver nanoparticles and their leishmanicidal potential against Indian strain of Leishmania donovani.
- Authors
Roy Chowdhury, Dibyapriya; Pramanik, Pijush Kanti; Ghosh, Priyanka; Devgupta, Pujayita; Chakraborti, Tapati
- Abstract
The present study was implemented to synthesize green silver nanoparticles from aqueous leaf extract of Nyctanthes arbor-tristis (AgNPNAs) and to analyze their physicochemical properties in evaluating their antileishmanial activity. Formation of AgNPNAs from leaf extract (LE) was evidenced by the change of peak intensity in the fluorescence spectrum. The particles were nearly monodispersed (PDI = 0.134) with hydrodynamic diameter of 67.83 nm and appeared quite stable (zeta potential = −19.1 mV). FESEM study demonstrated nearly spherical shape of AgNPNAs and apparently smooth surface area was identified by AFM. The diameter of AgNPNAs (~ 21 nm) was measured by HRTEM. The amorphous crystal nature, polycrystallinity and low grain size of AgNPNAs were comprehended by the XRD. The crystalline nature was supported from SAED profile. The EDX spectra identified the constituents of AgNPNAs whereas the XPS spectra unraveled the Ag2O state. The FTIR analysis implemented the phytofabricating agent(s) to be attached to the Ag0 via carboxyl group. The leishmanicidal activity of AgNPNAs on Leishmania donovani (MHOM/IN/1983/AG83) was compared with commercial silver nanoparticles (AgNPCOs), AgNO3, miltefosine and leaf extract (LE). The AgNO3 (IC50 = 0.469 ± 0.116 μg/ml), AgNPCOs (IC50 = 4.74 ± 0.221 μg/ml), miltefosine (IC50 = 0.87 ± 0.038 μg/ml) proved their better leishmanicidal efficacy than AgNPNAs (IC50 = 11.46 ± 0.120 μg/ml) and LE was found to be incompetent as parasiticidal. However, AgNPNAs showed greater safety level (IC50 = 136.33 ± 0.881 μg/ml) in normal macrophages (Raw 264.7) than AgNO3 (IC50 = 1.81 ± 0.133 μg/ml), AgNPCOs (IC50 = 10.06 ± 0.719 μg/ml) and miltefosine (IC50 = 18.73 ± 0.9 μg/ml). Altogether, this study revealed the physicochemical properties of AgNPNAs and their biocompatibility in killing L. donovani promastigotes indicating their new identity as potential antileishmanial agent.
- Subjects
LEISHMANIA donovani; MILTEFOSINE; FLUORESCENCE spectroscopy; ZETA potential; CARBOXYL group; SILVER nanoparticles; NANOPARTICLES analysis; MONODISPERSE colloids
- Publication
Applied Nanoscience, 2023, Vol 13, Issue 8, p5729
- ISSN
2190-5509
- Publication type
Article
- DOI
10.1007/s13204-023-02813-6