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- Title
METTL9 derived circular RNA circ-METTL9 sponges miR-551b-5p to accelerate colorectal cancer progression by upregulating CDK6.
- Authors
Li, Ming; Zhi, Zheng; Jiang, Xuan; Duan, Guo-Cai; Zhu, Wei-Na; Pang, Zheng; Wang, Lian; Ge, Rui; Dai, Xin; Liu, Jia-Meng; Chen, Ting-Yue; Jia, Jin-Jing; Li, Jian-Ming; Sun, Li-Na
- Abstract
Circular RNAs (circRNAs) have been accepted to play key roles in the development and progression of mutiple cancers including colorectal cancer (CRC). Here, we identified circ-METTL9, derived from 2 to 4 exons of METTL9 gene, may promote CRC progression by accelerating cell cycle progression. However, the role and mechanism of circ-METTL9 in CRC remains unclear. Based on our data, the expression of circ-METTL9 was significantly upregulated in CRC tissues and markedly increased in advanced tumors in CRC patients. Functional experiments demonstrated that circ-METTL9 overexpression promoted CRC cells proliferation and migration in vitro , and simultaneously enhanced CRC tumor growth and metastasis in vivo. Mechanistically, RNA immunoprecipitation (RIP) assays proved that circ-METTL9 might be a miRNA sponge, and RNA pulldown assays showed the interaction between circ-METTL9 and miR-551b-5p. Notably, cyclin-dependent kinase 6 (CDK6), a key regulator in cell cycle, is a conserved downstream target of miR-551b-5p. Taken together, our findings highlight a novel oncogenic function of circ-METTL9 in CRC progression via circ-METTL9/miR-551b-5p/CDK6 axis, which may serve as a prognostic biomarker and therapeutic target for CRC patients.
- Subjects
CIRCULAR RNA; COLORECTAL cancer; CANCER invasiveness; TUMOR growth; CELL cycle
- Publication
Carcinogenesis, 2023, Vol 44, Issue 6, p463
- ISSN
0143-3334
- Publication type
Article
- DOI
10.1093/carcin/bgad031