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- Title
Construction of recombinant fluorescent LSDV for high-throughput screening of antiviral drugs.
- Authors
Wang, Jingyu; Ji, Jinzhao; Zhong, Yongcheng; Meng, Wenxin; Wan, Shaobin; Ding, Xiaoqing; Chen, Zihan; Wu, Weiyong; Jia, Kun; Li, Shoujun
- Abstract
Lumpy skin disease virus (LSDV) infection is a major socio-economic issue that seriously threatens the global cattle-farming industry. Here, a recombinant virus LSDV-ΔTK/EGFP, expressing enhanced green fluorescent protein (EGFP), was constructed with a homologous recombination system and applied to the high-throughput screening of antiviral drugs. LSDV-ΔTK/EGFP replicates in various kidney cell lines, consistent with wild-type LSDV. The cytopathic effect, viral particle morphology, and growth performance of LSDV-ΔTK/EGFP are consistent with those of wild-type LSDV. High-throughput screening allowed to identify several molecules that inhibit LSDV-ΔTK/EGFP replication. The strong inhibitory effect of theaflavin on LSDV was identified when 100 antiviral drugs were screened in vitro. An infection time analysis showed that theaflavin plays a role in the entry of LSDV into cells and in subsequent viral replication stages. The development of this recombinant virus will contribute to the development of LSDV-directed antiviral drugs and the study of viral replication and mechanisms of action.
- Subjects
HIGH throughput screening (Drug development); ANTIVIRAL agents; LUMPY skin disease; HOMOLOGOUS recombination; GREEN fluorescent protein; RECOMBINANT viruses; AVIAN influenza
- Publication
Veterinary Research, 2024, Vol 55, Issue 1, p1
- ISSN
0928-4249
- Publication type
Article
- DOI
10.1186/s13567-024-01281-2