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- Title
A Novel Thermal Stable Carbonyl Reductase from Bacillus cereus by Gene Mining as Biocatalyst for β-Carbonyl Ester Asymmetric Reduction Reaction.
- Authors
Qin, Yan-Li; Ruan, Tao; Hou, He-Shuai; Hou, Ya-Li; Yang, Zhong-Hua; Quan, Can
- Abstract
Carbonyl reductase, as biocatalyst, is very important to chiral alcohols production through asymmetric reduction of carbonyl compound. A novel thermal stable carbonyl reductase from Bacillus cereus (BcCR) dependant on NADPH was obtained through a new genome mining strategy proposed in this work. By analyzing its amino acid sequence and structure, the BcCR should be a thermal stability and wide pH tolerance carbonyl reductase. Its gene was cloned by PCR with B. cereus genomic DNA as template. Its heterologous expression system, E. coli BL21 (DE3) plysS/pET28a-bccr, was constructed, and BcCR was successfully expressed. Enzymatic properties show that at 57.5 °C and pH 7.0 it can reach maximum reaction rate. Its Km and Vmax to ethyl 4-chloroacetoacetate is 1.85 mmol/L and 0.22 µmol/(min·mgprotein), respectively. It can catalyze the asymmetric reduction of the β-carbonyl compound, such as ethyl 4-chloroacetoacetate to ethyl S-4-chloro-3-hydroxybutyrate. This paper proposes a practical method for discovery of new carbonyl reductases, and provides a novel enzyme as biocatalyst for asymmetric reduction of β-carbonyl compound.
- Subjects
REDUCTASES; BACILLUS (Bacteria); GENES; ENZYMES; BACILLACEAE
- Publication
Catalysis Letters, 2019, Vol 149, Issue 2, p610
- ISSN
1011-372X
- Publication type
Article
- DOI
10.1007/s10562-018-2645-4