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- Title
Cortex-wide transcranial localization microscopy with fluorescently labeled red blood cells.
- Authors
Zhou, Quanyu; Glück, Chaim; Tang, Lin; Glandorf, Lukas; Droux, Jeanne; El Amki, Mohamad; Wegener, Susanne; Weber, Bruno; Razansky, Daniel; Chen, Zhenyue
- Abstract
Large-scale imaging of brain activity with high spatio-temporal resolution is crucial for advancing our understanding of brain function. The existing neuroimaging techniques are largely limited by restricted field of view, slow imaging speed, or otherwise do not have the adequate spatial resolution to capture brain activities on a capillary and cellular level. To address these limitations, we introduce fluorescence localization microscopy aided with sparsely-labeled red blood cells for cortex-wide morphological and functional cerebral angiography with 4.9 µm spatial resolution and 1 s temporal resolution. When combined with fluorescence calcium imaging, the proposed method enables extended recordings of stimulus-evoked neuro-vascular changes in the murine brain while providing simultaneous multiparametric readings of intracellular neuronal activity, blood flow velocity/direction/volume, and vessel diameter. Owing to its simplicity and versatility, the proposed approach will become an invaluable tool for deciphering the regulation of cortical microcirculation and neurovascular coupling in health and disease. Existing neuroimaging tools are still hampered by restricted field-of-view, slow imaging speed or suboptimal spatial resolution. Here, the authors present a fluorescence localization imaging approach aided by sparsely-labeled red blood cells for cortex-wide morphological and functional cerebral angiography with high spatiotemporal resolution.
- Subjects
ERYTHROCYTES; CEREBRAL angiography; MICROSCOPY; FLOW velocity; SPATIAL resolution; CEREBRAL circulation
- Publication
Nature Communications, 2024, Vol 15, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-024-47892-3