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- Title
Effect of Apatinib on Invasion and Migration of Lung Cancer Cells and Its Mechanism.
- Authors
Yin YUAN; Hao GONG; Yongwen LI; Hongbing ZHANG; Ying LI; Weiting LI; Pan WANG; Ruifeng SHI; Hongyu LIU; Jun CHEN
- Abstract
Background and objective Lung cancer is one of the most deadly cancers in the world for human. In recent years, the effect of targeted therapy has become increasingly significant. Apatinib is a multi-target anti-tumor drug that is currently under study. The purpose of this study is to investigate the effects of Apatinib on the biological characteristics of lung cancer cells and its possible mechanism. Methods Lung cancer cell lines H1299 and H3255 were cultured in vitro. The effects of Apatinib on proliferation, migration and invasion of H1299 and H3255 cells were detected by cell proliferation assays wound healing assays and Transwell assays. The protein expression related to cancer angiogenesis and invasion was detected by Western blot. Results Apatinib significantly inhibited the proliferation, migration and invasion of H1299 and H3255 in a concentration-dependent manner. Western blot showed that with the increasing of drug concentration, VEGF, VEGFR2, N-cadherin, MMP9, MMP2 and Vimentin were down-regulated, and E-cadherin were up-regulated. Conclusion Apatinib can inhibit the invasion and migration of lung adenocarcinoma cells H1299 and H3255. By regulation of epithelial-mesenchymal transition and the expression of matrix metalloproteinase-related proteins.
- Subjects
CELL proliferation; CELL adhesion molecules; CELL lines; CELL migration; CELL motility; CLINICAL trials; CYTOSKELETAL proteins; GROWTH factors; LUNG tumors; MICROBIOLOGICAL assay; WESTERN immunoblotting; PROTEIN-tyrosine kinase inhibitors; VASCULAR endothelial growth factors; IN vitro studies; PHARMACODYNAMICS; THERAPEUTICS
- Publication
Chinese Journal of Lung Cancer, 2019, Vol 22, Issue 5, p264
- ISSN
1009-3419
- Publication type
Article
- DOI
10.3779/j.issn.1009-3419.2019.05.02