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- Title
Microsatellite markers for Spergularia media (L.) C. Presl. (Caryophyllaceae) and their cross-species transferability.
- Authors
PRINZ, KATHLEEN; SCHIE, STEPHAN; DEBENER, THOMAS; HENSEN, ISABELL; WEISING, KURT
- Abstract
We developed 11 polymorphic microsatellite markers for Spergularia media (Caryophyllaceae), a perennial halophyte of coastal salt meadows and continental areas of western Eurasia. The number of alleles per locus observed in a single population of 20 individuals from the German North Sea coast ranged from 3 to 20. Observed and expected heterozygosities ranged from 0.200 to 0.850 and from 0.278 to 0.936 respectively. Observed heterozygosities were lower than expected heterozygosities at all loci, presumably as a consequence of inbreeding. All markers cross-amplified in the closely related S. salina. Of these, nine were polymorphic in the heterologous species. Only two primer pairs generated PCR products in S. diandra.
- Subjects
EURASIA; MICROSATELLITE repeats; SPERGULARIA media; HALOPHYTES; HETEROZYGOSITY; GENETIC polymorphisms
- Publication
Molecular Ecology Resources, 2009, Vol 9, Issue 5, p1424
- ISSN
1755-098X
- Publication type
Article
- DOI
10.1111/j.1755-0998.2009.02680.x