We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Linkage analysis of the resistance gene expressed dominantly against the Bt insecticide Cry1 Ac in the Silkworm Bombyx mor.
- Authors
Polan, Mohammad Sahin; Katsuhiko Ito; Kazuhisa Miyamoto; Takeshi Yokoyama; Osamu Ninagi
- Abstract
Resistance against Bacillus thuringiensis (Bt) toxin CrylAc in Bombyx mori is controlled by a resistance gene that is expressed dominantly. We developed a segregation model and determined the linkage group using linkage analysis with amplified fragment length polymorphism (AFLP) and single-nucleotide polymorphism (SNP) markers. We used the BF1 progeny produced by crossing female F1 (resistant c440 x susceptible N28) with male N28 strain, because no crossing over occurred in the female silkworms. To determine the segregation model, we screened c440, N28, F1, and BF1 by inoculating them with the commercial biopesticide Guardjet (7% CrylAc crystal protein). The LC50 value of c440 was 221.87 mg/ml, which was 2,017 times higher than that of N28 (LC50: 0.11 mg/ml). The LC50 value of F1 was 2.47 mg/ml, which was lower than that of c440 and higher than that of N28, indicating that this resistance mechanism is controlled by a resistance gene that express dominantly. For linkage analysis, we selected the CrylAc resistant BF1 individuals from two batches with the treatment of 3.13 mg/ml Guardjet, and designed primer sets, which can distinguish genotypes of c440 and N28 by AFLP or SNPs, for each chromosome. All resistant BF1 individuals should be heterozygous for the chromosome to which the resistance gene is linked. Linkage analysis revealed that all BF1 individuals were heterozygous for only chromosome 15, suggesting that the resistance gene Is linked to chromosome 15. We assigned this gene as "incompletely dominant resistance to CrylAc" and designated it as "Rc1ad".
- Subjects
SILKWORM diseases; BACILLUS thuringiensis genetics; DRUG resistance in bacteria; GENE expression in bacteria; SINGLE nucleotide polymorphisms; AMPLIFIED fragment length polymorphism
- Publication
Journal of Insect Biotechnology & Sericology, 2014, Vol 83, Issue 2, p33
- ISSN
1346-8073
- Publication type
Article