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- Title
RanGTPase links nucleo-cytoplasmic transport to the recruitment of cargoes into small extracellular vesicles.
- Authors
Chavan, Sakalya; Khuperkar, Deepak; Lonare, Akshay; Panigrahi, Swagatika; Bellare, Jayesh; Rapole, Srikanth; Seshadri, Vasudevan; Joseph, Jomon
- Abstract
Small extracellular vesicle (sEV)-mediated intercellular communication regulates multiple aspects of growth and development in multicellular organisms. However, the mechanism underlying cargo recruitment into sEVs is currently unclear. We show that the key nucleo-cytoplasmic transport (NCT) protein—RanGTPase, in its GTP-bound form (RanGTP), is enriched in sEVs secreted by mammalian cells. This recruitment of RanGTP into sEVs depends on the export receptor CRM1 (also called XPO1). The recruitment of GAPDH, a candidate cargo protein, into sEVs is regulated by the RanGTP–CRM1axis in a nuclear export signal (NES)-dependent manner. Perturbation of NCT through overexpression or depletion of nuclear transport components affected the recruitment of Ran, CRM1 and GAPDH into sEVs. Our studies, thus, suggest a link between NCT, particularly the Ran–CRM1 axis, and recruitment of NES-containing cargoes into the sEVs. Collectively, these findings implicate RanGTPase as a link between NCT and sEV mediated intercellular communication.
- Publication
Cellular & Molecular Life Sciences, 2022, Vol 79, Issue 7, p1
- ISSN
1420-682X
- Publication type
Article
- DOI
10.1007/s00018-022-04422-y