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- Title
Lymphocyte Activation IV. THE ULTRASTRUCTURAL PATTERN OF THE RESPONSE OF MOUSE T AND B CELLS TO MITOGENIC STIMULATION <em> IN VITRO</em>.
- Authors
Janossy, G.; Shohat, Maya; Greaves, M.F.; Dourmashkin, R.R.
- Abstract
Thymocytes from cortisone-treated mice ('T' cells), 'B' spleen cells (B lymphocytes from thyrnectomized, irradiated, marrow reconstituted mice) and normal spleen (T+B) cells were examined by electron microscopy after 60 hours stimulation by Concanavalin A (a T cell specific mitogen), endotoxin (B cell specific mitogen), and pokeweed mitogen (which stimulates both T and B cells). Stimulation oft cells by Con A or PWM induced the appearance of lymphoblasts (Type I) and only PWM or endotoxin stimulated B cells developed 'plasmablast' features (dilated, vesicular rough endoplasmic reticulum; Type II). A few stimulated B cells also had lymphoblast morphology. Large cells from normal (T+B) spleen stimulated by PWM were heterogeneous consisting of 55-60 per cent plasmablasts and 40-45 per cent lymphoblasts. It was concluded that the ultrastructure of stimulated lymphocytes depended on whether T or B cells were stimulated and not primarily on the mitogen used. In general, the response evoked by mitogens paralleled at the ultrastructural level that induced by antigens. It was also found that multivesicular bodies and glycogen particles occurred predominantly in the cytoplasm of stimulated T cells (lymphoblasts).
- Subjects
SPLEEN; LYMPHOCYTES; T cells; ELECTRON microscopy; ENDOTOXINS; IMMUNOLOGY
- Publication
Immunology, 1973, Vol 24, Issue 2, p211
- ISSN
0019-2805
- Publication type
Article