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- Title
Gelatin improves peroxidase‐mediated alginate hydrogel characteristics as a potential injectable hydrogel for soft tissue engineering applications.
- Authors
Morshedloo, Fatemeh; Khoshfetrat, Ali Baradar; Kazemi, Davoud; Ahmadian, Mehri
- Abstract
To develop an efficient injectable alginate‐based hydrogel for soft tissue engineering applications, phenol moiety (Ph) was introduced into alginate (Alg‐Ph), and the influence of gelatin as cell adhesive molecule was evaluated on the peroxidase‐mediated alginate hydrogel properties and cultured chondrocytic cell behavior. Addition of gelatin (1.5% w/v) to Alg‐Ph (1.5% w/v) hydrogels (Alg‐Ph/gelatin) regulated characteristics of the enzymatically gellable alginate hydrogel with increasing gelation time to 5.1 min (76%). Swelling ratio and degradation rates of the Alg‐Ph/gelatin hydrogel also increased 60 and 100%, respectively, while the mechanical strength value was 35% less than the Alg‐Ph hydrogel. Scanning electron microscopy images showed that the addition of gelatin could also increase uniformity of pore sizes inside the Alg‐Ph/gelatin hydrogels. The chondrocyte cells maintained their original phenotype and revealed statistically more metabolic activities in the Alg‐Ph/gelatin hydrogel. Hydrogels subscutaneously implanted in rats could also be identified readily without complete absorption and signs of toxicity or any untoward reactions after 1 month. Viable chondrocyte cells inside globular aggregates were seen as red colored areas in the cell‐laden hydrogels. The study demonstrates that enzymatically gellable alginate/gelatin hydrogel has fair potential as a natural‐based injectable hydrogel for soft tissue engineering applications.
- Subjects
TISSUE engineering; GELATIN; MOIETIES (Chemistry); SCANNING electron microscopy; ALGINIC acid
- Publication
Journal of Biomedical Materials Research, Part B: Applied Biomaterials, 2020, Vol 108, Issue 7, p2950
- ISSN
1552-4973
- Publication type
Article
- DOI
10.1002/jbm.b.34625