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- Title
CNS activity of Pokeweed Anti-viral Protein (PAP) in mice infected with Lymphocytic Choriomeningitis Virus (LCMV).
- Authors
Uckun, Fatih M.; Rustamova, Larisa; Vassilev, Alexei O.; Tibbles, Heather E.; Petkevich, Alexander S.
- Abstract
Background: Others and we have previously described the potent in vivo and in vitro activity of the broad-spectrum antiviral agent PAP (Pokeweed antiviral protein) against a wide range of viruses. The purpose of the present study was to further elucidate the anti-viral spectrum of PAP by examining its effects on the survival of mice challenged with lymphocytic choriomeningitis virus (LCMV). Methods: We examined the therapeutic effect of PAP in CBA mice inoculated with intracerebral injections of the WE54 strain of LCMV at a 1000 PFU dose level that is lethal to 100% of mice within 7-9 days. Mice were treated either with vehicle or PAP administered intraperitoneally 24 hours prior to, 1 hour prior to and 24 hours, 48 hours 72 hours and 96 hours after virus inoculation. Results: PAP exhibits significant in vivo anti- LCMV activity in mice challenged intracerebrally with an otherwise invariably fatal dose of LCMV. At non-toxic dose levels, PAP significantly prolonged survival in the absence of the majority of disease-associated symptoms. The median survival time of PAP-treated mice was >21 days as opposed to 7 days median survival for the control (p =0.0069). Conclusion: Our results presented herein provide unprecedented experimental evidence that PAP exhibits antiviral activity in the CNS of LCMV-infected mice.
- Subjects
ANTIVIRAL agents; CENTRAL nervous system; POKEWEED; PROTEINS; LYMPHOCYTIC choriomeningitis virus; LABORATORY mice
- Publication
BMC Infectious Diseases, 2005, Vol 5, Issue 1, p9
- ISSN
1471-2334
- Publication type
Article
- DOI
10.1186/1471-2334-5-9