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- Title
DETERMINING THE POSITION OF THE ENZYME ALKALINE PHOSPHATASE BY HISTOLOGICAL METHODS.
- Authors
Kaminoff, Harvey
- Abstract
The article presents information on the use of histological methods to determine the position of alkaline phosphatase enzyme in the cell. An enzyme is an organic catalyst that changes the starch to sugar. Alkaline phosphatase has the job of changing certain compounds of phosphoric acid called esters, into phosphates. The rat kidneys were the tissues used, they were fixed in 80 percent alcohol for about 8 to 24 hours. They were dehydrated in 100 percent alcohol for about 24 hours, and then cleared in cedarwood oil, embedded in paraffin and sectioned. The sections were then incubated in the solution that contains a compound of phosphoric acid, namely, sodium glycerophosphate, and it is upon this compound that the enzyme acts. After 24 hours of incubation, the slides were immersed in cobalt nitrate, which changed the calcium phosphate to cobalt phosphate. Next, the sections were treated with ammonium sulfide. Finally, the sections are mounted in canada balsam. By this technique, the prepared slide of a section of a kidney stained to show where the enzyme alkaline phosphatase is located.
- Subjects
HISTOLOGICAL techniques; ALKALINE phosphatase; ZINC enzymes; CORROSION casting (Microscopy); PROTEINS; CATALYSTS; PHOSPHORIC acid; TISSUES; LABORATORY rats
- Publication
Science Education, 1948, Vol 32, Issue 1, p43
- ISSN
0036-8326
- Publication type
Article
- DOI
10.1002/sce.3730320115