We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
ARSENIC-INDUCED STEATOSIS IN C57BL/6J MALE MICE CHRONICALLY EXPOSED THROUGH DRINKING WATER.
- Authors
Vigo, Jaime B.; Ellzey, Joanne T.; Baker, Thomas P.; Bader, Julia O.; Oliver, Thomas G.
- Abstract
Epidemiological evidence suggests that chronic ingestion of arsenic through drinking water may have links with the onset of diabetes mellitus-Type 2. This investigation used an in vivo experimental setting to test the hypothesis that chronic ingestion of arsenic through the drinking water may induce diabetogenic effects in a mouse model fed with a low fat or a high fat diet. Four groups of C57BL/6J male mice (n=15/group) were fed with 4.5% (low) or 11% (high) fat diet, and exposed to drinking water containing 0 ppm or 22.5 ppm of sodium arsenite [As (III)] for 40 weeks. During the last 10 weeks, the fat content of the 11% fat diet groups was increased to 24% to accelerate increase in weight gain and to ensure reaching the obesity threshold (=50 g) within the experimental timeline. Light and transmission electron microscopy studies revealed an arsenic-induced hepatic steatosis and inflammation in the As (III)-exposed low fat group compared to the control low-fat group. A similar diet-induced hepatic steatosis and inflammation were also observed in both of the high fat diet groups with no significantly different pathology observed between the arsenic-exposed and control groups. A significant diet-induced increase in the average accumulated weights starting at week 6 (diet by week interaction p<0.001) and blood glucose of the groups fed with the high fat diet (p<0.001) compared to the low fat diet groups were observed. Terminal blood insulin showed a significant diet-induced increase for the high fat fed groups compared to the low fat fed groups (p=0.02). The organ to body weight ratio showed a significant diet effect for the liver (p<0.001), a significant diet effect (p=0.005) and a significant group effect (p=0.03) for the kidney, and a significant diet effect (p=0.01) for pancreas. There were significant group*diet*week interactions in both the amount of average water consumption and average cumulative food consumption. No significant differences were found for BUN. In conclusion, 40 weeks of exposure of C57BL/6J mice to 22.5 ppm of As (III) through drinking water did not provide evidence of diabetogenic effects but did provide evidence of steatosis in lean mice due to arsenic toxicity.
- Subjects
ARSENIC compounds; FATTY degeneration; MICE; INFLAMMATION; FIBROSIS; DRINKING water; ELECTRON microscopy
- Publication
Texas Journal of Microscopy, 2007, Vol 38, Issue 2, p117
- ISSN
1554-0820
- Publication type
Article