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- Title
Avidin conjugation to up-conversion phosphor NaYF<sub>4</sub>:Yb<sup>3+</sup>, Er<sup>3+</sup> by the oxidation of the oligosaccharide chains.
- Authors
Zewei Quan; Jun Yang; Piaoping Yang; Chunxia Li; Jun Lin
- Abstract
Abstract NaYF4:Yb3+, Er3+ nanoparticles were successfully prepared by a polyol process using diethyleneglycol (DEG) as solvent. After being functionalized with SiO2–NH2 layer, these NaYF4:Yb3+, Er3+ nanoparticles can conjugate with activated avidin molecules (activated by the oxidation of the oligosaccharide chain). The as-formed NaYF4:Yb3+, Er3+ nanoparticles, NaYF4:Yb3+, Er3+ nanoparticles functionalized with amino groups, avidin conjugated amino-functionalized NaYF4:Yb3+, Er3+ nanoparticles were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), atomic force microscopy (AFM), Fourier transform infrared (FT-IR), UV/Vis absorption spectra, and up-conversion luminescence spectra, respectively. The biofunctionalization of the NaYF4:Yb3+, Er3+ nanoparticles has less effect on their luminescence properties, i.e., they still show the up-conversion emission (from Er3+, with 4S3/2 → 4I15/2 at ~540 nm and 4F9/2 → 4I15/2 at ~653 nm), indicative of the great potential for these NaYF4:Yb3+, Er3+ nanoparticles to be used as fluorescence probes for biological system.
- Subjects
BIOCONJUGATES; AVIDIN; METAL clusters; OXIDATION; OLIGOSACCHARIDES; PHOSPHORS; SOLVENTS; X-ray diffraction
- Publication
Journal of Nanoparticle Research, 2009, Vol 11, Issue 4, p821
- ISSN
1388-0764
- Publication type
Article