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- Title
Irradiated stem cells and ageing of the haematopoietic system.
- Authors
Vávrová, Jiřina; Šinkorová, Zuzana; Řezáčová, Martina; Tichý, Aleš; Filip, Stanislav; Mokrý, Jaroslav; Lukášová, Emilie
- Abstract
In the work presented here, changes in haematopoiesis of mice (B6129SF2/J) were studied 1 year after their whole-body exposure to a dose of 7 Gy (72% of mice survived). The irradiated mice were compared with non-irradiated younger (4 months of age) and older (16 months of age) mice. There was a significant increase in the relative abundance of primitive stem cells with long-term capability of the haematopoiesis recovery lin/Sca-1/CD117/CD34 in the bone marrow of mice aged 16 months (irradiated and non-irradiated) compared with those aged 4 months. In terms of the ability to respond to further whole-body irradiation at a dose of 1 Gy, the presence of γH2A.X foci was studied in lin bone marrow cells. There was a considerable number of persisting foci in lin stem cells isolated from the bone marrow of the older irradiated mice. In the blood count from the peripheral blood of the older mice (both non-irradiated and irradiated at 7 Gy), there was a significant increase in granulocytes. In the group exposed to 7 Gy, the numbers of thrombocytes significantly increased, and on the contrary, the numbers of erythrocytes, the amount of haemoglobin, and haematocrit significantly decreased.
- Subjects
STEM cells; HEMATOPOIESIS; LABORATORY mice; DOSE-response relationship (Radiation); IRRADIATION
- Publication
Radiation & Environmental Biophysics, 2012, Vol 51, Issue 2, p205
- ISSN
0301-634X
- Publication type
Article
- DOI
10.1007/s00411-012-0401-5