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- Title
Envelopment by endothelial cells initiates translocation of avian primordial germ cell into vascular tissue.
- Authors
Murai, Hidetaka; Shibuya, Minami; Kishita, Ryohei; Sunase, Chihiro; Tamura, Koji; Saito, Daisuke
- Abstract
Background: In avian species, primordial germ cells (PGCs) migrate to the gonadal primordium through the vascular system. Because this mode of migration is reminiscent of cancer metastasis, it would be useful to elucidate the mechanisms underlying PGC migration via the bloodstream. Here, we sought to determine when, where, and how PGCs enter the vascular network by double visualization of PGCs and endothelial cells (ECs) in tie1:H2B‐eYFP transgenic quails. Results: In the left and right lateral germinal crescent regions corresponding to the anterior‐most area vasculosa, more than 60% of PGCs were enveloped by differentiating ECs forming blood islands prior to vascular network formation. Cell morphology analysis suggested that the PGC‐EC interaction was instructed by differentiating ECs. At a later developmental stage, ECs anastomosed to form a vascular network with a lumen that retained PGCs within it. As a consequence, many PGCs localized within the luminal space of the mature vascular network at later stages. Conclusions: Our findings demonstrate that the major type of avian PGC translocation into vascular tissue is not a typical intravasation, as performed by types of metastatic cancer cells, but rather a passive translocation (envelopment) mediated by differentiating ECs during early vasculogenesis. Key Findings: PGCs are adjacent to EC lineage cells in lateral germinal crescent regions of quail embryo from the early developmental stage.PGCs are enveloped by ECs in the extraembryonic region prior to vascular network formation.QH1 immunoreactivity on PGCs changes during development.
- Subjects
GERM cells; ENDOTHELIAL cells; CELL morphology; CELL analysis; CARDIOVASCULAR system
- Publication
Developmental Dynamics, 2021, Vol 250, Issue 10, p1410
- ISSN
1058-8388
- Publication type
Article
- DOI
10.1002/dvdy.332