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- Title
Effects of Growth Regulators, Organic Nitrogen Sources, Various Nutrient, and Non-Nutrient Adjuvants on Somatic Embryogenesis in Red Sandalwood (Pterocarpus Santalinus).
- Authors
Chakraborty, Tanushree; Chaitanya, K. Viswanatha; Akhtar, Nasim
- Abstract
The effects of growth regulators, organic nitrogen sources (amino acids and casein hydrolysate), coconut water, polyethylene glycol, dexamethasone, putrescine, sodium nitroprusside, and calcium chloride were studied on somatic embryogenesis and plantlet regeneration in Pterocarpus santalinus using immature zygotic embryo. The maximum frequency (83.33%) of embryogenesis, number of globular-staged embryos (88.00), number of cotyledonary-staged embryos (10.66), and number of plantlets (8.66) were obtained at 4 mg/l 2,4-D (2,4-Dichlorophenoxyacetic acid) + 0.1 mg/l BAP (6-Benzylaminopurine). Amino acids (proline, glutamine, tryptophan, and phenylalanine) and polyethylene glycol enhanced somatic embryogenesis. The addition of proline, glutamine, tryptophan, and phenylalanine at 100 mg/l each + 2% polyethylene glycol with 4 mg/l 2,4-D + 0.1 mg/l BAP resulted in maximum embryogenic frequency (100%), number of globular-staged embryos (155.00), number of cotyledonary-staged embryos (33.33), and number of plantlets regenerated (15.33). The plantlets obtained were acclimatized well and survived 100% in laboratory and greenhouse environments. The non-embryogenic and embryogenic development were discerned by histological examination. The study might have prospects concerning molecular studies and large-scale multiplication of P. santalinus or other endangered legume woody species. Key Message: 2,4-D, BAP, amino acids, and polyethylene glycol significantly affected somatic embryogenesis in Pterocarpus santalinus.
- Subjects
SOMATIC embryogenesis; GROWTH regulators; POLYETHYLENE glycol; AMINO acids; CALCIUM chloride; COCONUT water
- Publication
Journal of Plant Growth Regulation, 2024, Vol 43, Issue 4, p1026
- ISSN
0721-7595
- Publication type
Article
- DOI
10.1007/s00344-023-11157-y