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- Title
Genetic analysis of basophil function in vivo.
- Authors
Sullivan, Brandon M; Liang, Hong-Erh; Bando, Jennifer K; Wu, Davina; Cheng, Laurence E; McKerrow, James K; Allen, Christopher D C; Locksley, Richard M
- Abstract
Contributions by basophils to allergic and helminth immunity remain incompletely defined. Using sensitive interleukin 4 (Il4) reporter alleles, we demonstrate here that basophil IL-4 production occurs by a CD4+ T cell-dependent process restricted to the peripheral tissues affected. We genetically marked and achieved specific deletion of basophils and found that basophils did not mediate T helper type 2 (TH2) priming in vivo. Two-photon imaging confirmed that basophils did not interact with antigen-specific T cells in lymph nodes but engaged in prolonged serial interactions with T cells in lung tissues. Although targeted deletion of IL-4 and IL-13 in either CD4+ T cells or basophils had a minimal effect on worm clearance, deletion from both lineages demonstrated a nonredundant role for basophil cytokines in primary helminth immunity.
- Subjects
BASOPHILS; HELMINTHS; IMMUNITY; INTERLEUKIN-4; GENE frequency; T cells; ANTIGENS; CYTOKINES
- Publication
Nature Immunology, 2011, Vol 12, Issue 6, p527
- ISSN
1529-2908
- Publication type
Article
- DOI
10.1038/ni.2036