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- Title
Anticancer Property Of L-Glutaminase Producing Actinomycete Streptomyces Albogriseolus Isolated From Estuary Of Uttara Kannada District Against Hela And HepG2 Cell Lines.
- Authors
Mesta, Sunita C.; Onkarappa, R.
- Abstract
L-glutaminase (L-glutamine aminohydrolase EC 3.5.1.2) is an extracellular hydrolytic enzyme having anticarcinogenic potential and is widely used in enzyme therapy especially for acute lymphocytic leukemia. L-glutaminase deaminates L-glutamine to glutamic acid and ammonia. In most tumors glutamine is the primary mitochondrial substrate and is present in circulating blood. It is a source of essential amino acid necessary for development of leukemic cells. The lack or depletion of L-glutamine leads to death of tumour cells. The marine environment is a potential source of bioactive secondary metabolites that provides pharmaceutically important compounds. The present study was focussed on to study the anticancer property of L-glutaminase producing actinomycetes isolated from estuaries of Uttara Kannada district of Karnataka. The isolates were screened for L-glutaminase production by qualitative and quantitative assay and the potent isolate was further subjected for MTT assay to determine its anticancer potential. The study showed 60% of isolates were positive for L-glutaminase production in Rapid plate assay and 85% of isolates were positive in semiquantitative assay. In quantitative assay the isolate Streptomyces albogriseolus exhibited high enzyme activity of 24.32+0.02 IU/ml. In MTT assay the isolate Streptomyces albogriseolus showed an IC50 value 102.0μg/ml in cervical cancer HeLa cells and IC50 value of 101.2μg/ml in HePG2 cells respectively, and could be used as good source of L-glutaminase.
- Subjects
KARNATAKA (India); HELA cells; STREPTOMYCES; LYMPHOBLASTIC leukemia; ESSENTIAL amino acids; CELL lines
- Publication
Journal of Advanced Zoology, 2024, Vol 45, Issue 3, p15
- ISSN
0253-7214
- Publication type
Article