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- Title
MEIOTIC COMPETENCE OF BOVINE OOCYTES INFLUENCES MITOCHONDRIAL GENE EXPRESSION DURING IN VITRO MATURATION.
- Authors
HULINSKA, P.; NEMCOVA, L.; JESETA, M.; HANZALOVA, K.; TRAVNICKOVA, I.; KANKA, J.; MACHATKOVA, M.
- Abstract
For the in vitro studies, oocytes are usually collected from antral follicles independently of the follicular development stage. Such heterogeneous population of the oocytes differs in meiotic and developmental potential. It is generally accepted that the meiotic and developmental competencies of bovine oocytes can be expressed by both size and health of follicles from which the oocytes are recovered. The objectives of the present study were to assess the changes in expression of mRNA transcripts playing an important role in mitochondrial DNA metabolism during in vitro maturation of bovine oocytes with different meiotic competence. Meiotically more and less competent oocytes were obtained separately either from medium (MF) or small (SF) follicles, categorized into healthy and light-atretic categories according to oocyte morphology and matured using a standard protocol. They were examined at 0 and 24 hours of maturation. Either total RNA or poly(A) RNA was extracted from oocytes and the evaluation of gene expression of three mitochondrial factors (TFAM, TFB1M, and TFB2M), MATER, and Luciferase, as external standard, was performed by an RT-PCR. The level of TFAM, TFB1M and MATER poly(A) RNA transcripts significantly decreased in both MF and SF oocytes after maturation compared with that before maturation. The healthy MF and SF oocytes contained significantly higher amounts of TFB1M at the GV stage than light-atretic oocytes. However, no differences in the transcript abundance were found among these categories at the MII stage. While in healthy and light-atretic MF oocytes TFB2M abundance revealed no differences among the GV and MII stage, in SF oocytes TFB2M was increased at the MII stage. In light-atretic SF oocytes the increase was significant (p = 0.05) The level of TFAM total RNA significantly increased after maturation compared with that before maturation in all oocyte categories. On the other hand, no significant differences in the transcript abundance were found for TFB1M, TFB2M and MATER total RNA between the GV and MII stages in any oocyte category. The significantly higher amount of MATER total RNA was detected only in healthy MF oocytes in comparison with that in light-atretic MF oocytes after maturation. It can be concluded that differences in mitochondrial genes, MATER poly(A) and total RNA expression were revealed among the maturing healthy and light-atretic meiotically more and less competent bovine oocytes.
- Subjects
GENE expression; DNA metabolism; MITOCHONDRIAL DNA
- Publication
Slovak Journal of Animal Science, 2018, Vol 51, Issue 4, p171
- ISSN
1337-9984
- Publication type
Abstract