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- Title
In vitro PFOS exposure on immune endpoints in bottlenose dolphins ( Tursiops truncatus) and mice.
- Authors
Wirth, Jena R.; Peden‐Adams, Margie M.; White, Natasha D.; Bossart, Gregory D.; Fair, Patricia A.
- Abstract
ABSTRACT Previous studies in our lab have shown that perfluorooctane sulfonate (PFOS) modulates immune function in mice and correlates with many immune parameters in bottlenose dolphins ( Tursiops truncatus). In this study, bottlenose dolphin peripheral blood leukocytes (PBLs) and adult female B6C3F1 mouse splenocytes were exposed to environmentally relevant PFOS concentrations (0-5 µg ml-1) in vitro; and natural killer (NK) cell activity and lymphocyte proliferation (T and B cell) were assessed using the parallelogram approach for risk assessment. The objectives were: to corroborate results from the correlative studies in bottlenose dolphins with in vitro PFOS exposures; to evaluate the sensitivity of the mouse model as compared with bottlenose dolphins; and to assess risk using the parallelogram approach. In mouse cells, NK cell activity was decreased at in vitro doses of 0.01, 0.5, 0.1, 0.5 and 1 µg PFOS ml-1 and increased at 5 µg ml-1. Additionally, B cell proliferation was not altered, but T cell proliferation was decreased at all in vitro PFOS exposures. In dolphin cells, NK cell activity and T cell proliferation were not altered by in vitro PFOS exposure, but B cell proliferation exhibited a positive association in relation to PFOS dose. Overall, the data indicates that: the in vitro exposures of bottlenose dolphin PBLs exhibited results similar to reported correlative fields studies; that mice were generally more sensitive (for these selected endpoints) than were dolphins; and that the parallelogram approach could be used two-thirds of the time to predict the effects in bottlenose dolphins. Copyright © 2013 John Wiley & Sons, Ltd.
- Subjects
BOTTLENOSE dolphin; LEUCOCYTES; LYMPHOCYTES; PARALLELOGRAMS; KILLER cells
- Publication
Journal of Applied Toxicology, 2014, Vol 34, Issue 6, p658
- ISSN
0260-437X
- Publication type
Article
- DOI
10.1002/jat.2891