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- Title
RESEARCH PAPER Assessment method for allelopathic effect from leaf litter leachates.
- Authors
Fujii, Yoshiharu; Shibuya, Tomoko; Nakatani, Keiko; Itani, Tomio; Hiradate, Syuntaro; Parvez, Mohammad M.
- Abstract
In order to elucidate the allelopathic effect of leaf litter leachates under laboratory conditions, a modified ‘sandwich method’, which places leaves between two layers of agar, was used. Fifty mg of leaves was used per 10 cm2 cell. Agar concentrations at 0.5–1.0% were the best for gel support in determining radicle and hypocotyl elongation of lettuce. The optimum incubation time for bioassay was three days after imbibition onset. Among 20 typical tree species in Asia, Cymbopogon citratus and Derris scandens showed the strongest inhibitory activity determined by the sandwich method, followed by Piper betle, Tamarindus indica, and Gliricidia sepium. This bioassay seems to be a reliable method for screening allelopathic activity from leaf litter leachates.
- Subjects
ALLELOPATHY; ALLELOPATHIC agents; PLANT growth inhibiting substances; BACTERIAL leaching; LEACHATE; PLANT physiology; PLANT ecology
- Publication
Weed Biology & Management, 2004, Vol 4, Issue 1, p19
- ISSN
1444-6162
- Publication type
Article
- DOI
10.1111/j.1445-6664.2003.00113.x