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- Title
Effects of relaxin and IGF-I on capacitation, acrosome reaction, cholesterol efflux and utilization of labeled and unlabeled glucose in porcine spermatozoa.
- Authors
MIAH, ABDUL GAFFAR; SALMA, UMMAY; TAKAGI, YUJI; KOHSAKA, TETSUYA; HAMANO, KO-ICHI; TSUJII, HIROTADA
- Abstract
Aim: Relaxin and insulin-like growth factor (IGF)-I have pronounced effects on the male and female reproductive tracts. The aim of this study was to investigate the effects of relaxin and IGF-I on the motility, capacitation, acrosome reaction, cholesterol efflux and utilization of glucose in porcine spermatozoa. Methods: Swim-up separated spermatozoa that had been washed twice were incubated at 37°C for 1 or 4 h in modified Tyrode's albumin lactate pyruvate (mTALP) medium supplemented without (control) or with relaxin (20 ng/mL) or IGF-I (20 ng/mL) or both (10 + 10 ng/mL). Results: Progressive motility and the induction rate of capacitation and acrosome reaction were increased ( P < 0.05) by relaxin and IGF-I alone or in combination, especially after 4 h of incubation. Relaxin alone or combined with IGF-I enhanced ( P < 0.05) the cholesterol efflux after 4 h, whereas IGF-I alone did not show any significant effect on the cholesterol efflux compared with the control at any time point. The utilization rates of labeled and unlabeled glucose increased ( P < 0.05) in spermatozoa incubated with relaxin or IGF-I alone or in combination compared with the control. Conclusion: Thus, supplementation of relaxin alone or combined with IGF-I into the medium possibly plays a beneficial role in porcine spermatozoal prefertilization events in vitro. (Reprod Med Biol 2008; 7: 29–36)
- Subjects
ACROSOME reaction; SOMATOMEDIN; SPERM motility; RELAXIN; PORCINE somatotropin; GROWTH factors; CHOLESTEROL
- Publication
Reproductive Medicine & Biology, 2008, Vol 7, Issue 1, p29
- ISSN
1445-5781
- Publication type
Article
- DOI
10.1111/j.1447-0578.2007.00198.x