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- Title
高效液相色谱法测定淫羊藿药材及饮片中朝藿定A,朝藿定B,朝藿定C和淫羊藿苷的含量
- Authors
王世杰; 高云佳; 周书繁; 韩志岭; 张文涛; 李为理; 姜勇
- Abstract
OBJECTIVE: To establish the method for simultaneous determination of epimedins A, B and C and icariin in epimedii folium medicinal materials and decoction pieces by HPLC. METHODS: The samples were separated on an Agilent Zorbax SB-C18(4. 6 mm x 250 mm, 5 µm) column, with mobile phase of 0.01% acetonitrile with phosphoric acid water (V: V= 24 : 76 ) ; the flow rate was 1.0 ml/min; the column temperature was 30 °C ; the wavelength was 270 nm. RESULTS; The linear ranges for epimedins A, B and C and icariin were respectively 0. 068-0.408, 0.316-1.896, 0.280-2.240 and 0.434-2.604 µg, with average average recoveries of 103. 49% , 98. 80% , 96. 75% and 102. 10%. CONCLUSIONS: The established HPLC quantitative method is quick and accurate, and can be used to control the quality of epimedii folium medicinal materials and decoction pieces.
- Publication
Evaluation & Analysis of Drug-Use in Hospitals of China, 2018, Vol 18, Issue 2, p156
- ISSN
1672-2124
- Publication type
Article
- DOI
10.14009/j.issn.1672-2124.2018.02.003